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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant position in advertising and marketing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This information outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This information outlines even more achievable optimization of mobile and immunoassay parameters to ensure the very best effects are...
Antibodies certain to the blocking peptide now not bind into the epitope over the goal protein. By evaluating the staining in the blocked antibody as opposed to the antibody on get more info your own, you can see which staining is precise.
Goal Information and facts Cyclin E belongs for the really conserved cyclin family, whose users are characterised by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a complex with and features as a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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